Mar 10, 2020

Associate Scientist II/III, Gene Therapy - Vector Production

  • Professional Diversity Network
  • Cambridge, MA, USA
Full-Time Research

Job Description

Biogen

Job Description

The Gene Therapy Accelerator Unit (GTxAU) at Biogen is seeking a committed, enthusiastic, and dependable scientist to contribute to the generation of gene therapy clinical candidates for neurodegenerative and neuroinflammatory diseases.

The Associate Scientist II/III, Vector Production, will work collaboratively with a cross-function team and has high growth potentials. The incumbent will be part of a team focused on the production and purification of novel AAV vectors for in vitro and in vivo testing. The ideal candidate should have extensive experience in mammalian cell culture, cell line engineering, protein/viral vector purification, and biochemical/biophysical assays. He/She should be able to design, execute, and analyze in vitro experiments independently with strong scientific rigor. The ideal candidate should also maintain an up-to-date knowledge of pertinent literature and possess excellent communication and interpersonal skills to efficiently present results to project team members.

  • AAV packaging using triple transfection or producer cell lines to support internal research
  • AAV purification using ultracentrifugation and protein chromatography
  • AAV vector characterization by adapting existing protocols, including titering assay by ddPCR, genome integrity assay, empty/full particle ratio assay, endotoxin assay, residual DNA and protein assays
  • Maintain constant awareness of novel biochemical and biophysical technologies for AAV purification and characterization
  • Work as a liaison between GTxAU and the upstream and downstream process teams
  • Establish SOPs and adapt new protocols from literature
  • Manage external CROs for plasmid or vector production
  • Maintain electronic laboratory notebooks, present data to the team, and write study reports

#LI-SK1

Qualifications

3+ years of experience in the biotech/pharmaceutical industry
Strong skillsets in mammalian cell culture and cell line engineering
Hands-on experience with protein or viral vector purification techniques (Ultracentrifugation and chromatography)
Hands-on experience with molecular biology techniques (cloning, qPCR, ddPCR, DNA gel electrophoresis, SDS-PAGE, WB, ELISA, etc.)
Ability to independently design, execute, and analyze in vitro molecular biology experiments
Critical thinking and presentation skills a must
Strong communication and organizational skills
Ability to productively collaborate in cross-function teams

Preferred:
Hands-on experience with AAV and/or other gene therapy delivery systems
Hands-on experience with AUC
Previous research experience in neuroscience/neurological disorders

Education:
BS or MS in scientific field (biology, biochemistry, biotechnology, etc.)

Additional Information

The Associate Scientist II/III, Gene Therapy - Vector Production, will work collaboratively with a cross-function team and has high growth potentials. The incumbent will be part of a team focused on the production and purification of novel AAV vectors for in vitro and in vivo testing.

States

MA  

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