Associate Scientist II/III, Gene Therapy - Vector Production
Professional Diversity Network
Cambridge, MA, USA
The Gene Therapy Accelerator Unit (GTxAU) at Biogen is seeking a committed, enthusiastic, and dependable scientist to contribute to the generation of gene therapy clinical candidates for neurodegenerative and neuroinflammatory diseases.
The Associate Scientist II/III, Vector Production, will work collaboratively with a cross-function team and has high growth potentials. The incumbent will be part of a team focused on the production and purification of novel AAV vectors for in vitro and in vivo testing. The ideal candidate should have extensive experience in mammalian cell culture, cell line engineering, protein/viral vector purification, and biochemical/biophysical assays. He/She should be able to design, execute, and analyze in vitro experiments independently with strong scientific rigor. The ideal candidate should also maintain an up-to-date knowledge of pertinent literature and possess excellent communication and interpersonal skills to efficiently present results to project team members.
AAV packaging using triple transfection or producer cell lines to support internal research
AAV purification using ultracentrifugation and protein chromatography
AAV vector characterization by adapting existing protocols, including titering assay by ddPCR, genome integrity assay, empty/full particle ratio assay, endotoxin assay, residual DNA and protein assays
Maintain constant awareness of novel biochemical and biophysical technologies for AAV purification and characterization
Work as a liaison between GTxAU and the upstream and downstream process teams
Establish SOPs and adapt new protocols from literature
Manage external CROs for plasmid or vector production
Maintain electronic laboratory notebooks, present data to the team, and write study reports
3+ years of experience in the biotech/pharmaceutical industry Strong skillsets in mammalian cell culture and cell line engineering Hands-on experience with protein or viral vector purification techniques (Ultracentrifugation and chromatography) Hands-on experience with molecular biology techniques (cloning, qPCR, ddPCR, DNA gel electrophoresis, SDS-PAGE, WB, ELISA, etc.) Ability to independently design, execute, and analyze in vitro molecular biology experiments Critical thinking and presentation skills a must Strong communication and organizational skills Ability to productively collaborate in cross-function teams
Preferred: Hands-on experience with AAV and/or other gene therapy delivery systems Hands-on experience with AUC Previous research experience in neuroscience/neurological disorders
Education: BS or MS in scientific field (biology, biochemistry, biotechnology, etc.)
The Associate Scientist II/III, Gene Therapy - Vector Production, will work collaboratively with a cross-function team and has high growth potentials. The incumbent will be part of a team focused on the production and purification of novel AAV vectors for in vitro and in vivo testing.